PARASITES IN MALARIAL FEVER ;2y 



eight to fourteen days, the young amoeboid forms of the 

 parasite will be found in the man so injected if he be 

 susceptible. 



Stained Films. In blood films stained by Leishman's 

 method the various stages can also conveniently be 

 studied, and certain points in the cell structure can only 

 be brought out in such specimens. 



Human Phase 



Endogenous 

 or Asexual Cycle 



The 

 Mosquito Phase 



Exogenous 

 OF Sexual Cycle, 



FIG. 9. Development of malarial parasite. The exogenous cycle requires 

 an alternative host a mosquito, and the zygote is at first motile, ookinet. 



Leishman's stain is practically a triple stain ; it contains 

 eosin, which has a special affinity for formed material but 

 not for parts of cells actively concerned in growth or 

 reproduction. It is a so-called acid stain, and stains 

 the haemoglobin in blood and some of the granules in 

 certain leucocytes. It stains faintly the protoplasm of 

 some of the leucocytes. The methylene blue has been 

 altered by the polychroming so that two stains are present, 



