LOUIS FREEDMAN 13 



Our results as detailed in table IV, show that we can obtain 

 from autolyzed yeast by fractional adsorption with fullers earth 

 and norit, and subsequent extraction of the adsorbents, a con- 

 centrated solution of vitamine D, almost free from vitamine B. 

 This vitamine D extract is active in stimulating the growth of 

 both yeast cells and streptococci, but is not curative for beriberi. 

 We were, however, unable to obtain vitamine B entirely free 

 from D as this fraction which was found to be curative for beri- 

 beri was also active in stimulating the growth of yeast cells and 

 bacteria. We have also shown that the extraction of these active 

 substances from the adsorbents with baryta and acetic acid is 

 almost quantitative. 



SHAKING OF BEEF-HEART INFUSIONS WITH FULLERS EARTH AND 



NORIT. 



A beef-heart infusion, prepared as described in the early 

 part of this paper, when shaken for three hours with 50 grams 

 of fullers earth per liter, lost most of its stimulating effect on 

 yeast growth, while it still retained enough of the active sub- 

 stance to promote the growth of streptococci. The nitrate, after 

 a second shaking with fullers earth, (100 grams per liter) 

 showed no activity on the growth of either yeast or bacteria. 



The baryta extract of the fullers earth from the first shak- 

 ing, peculiarly showed only a very slight growth-stimulating 

 activity on yeast; even the addition of 2 cc. of the extract to 

 the medium increased the growth of yeast cells only slightly. 

 The baryta extract of the fullers earth from the second shaking 

 was correspondingly less active on yeast growth, showing a net 

 growth of only 1 mm. ; while it was only slightly active on bac- 

 terial growth. The effects on the two organisms, however, were 

 comparable, as shown in table V. 



Norit removed from beef-heart infusions practically all of 

 the substance which stimulates the growth of yeast when the 

 infusion was shaken with 2% of its weight (approximately 20 

 grams per liter) for three hours. The infusion was, however, 

 still effective for the growth of streptococci. A second shaking, 

 this time with 50 grams per liter, removed all activity on yeast 

 and bacterial growth. 



The acetic acid extract of the norit from the first shaking 

 showed great growth stimulation on both organisms, this ex- 

 tract giving almost as much stimulation as the original me- 

 dium. The net growth stimulating activity of the extract on 



