AM(EBA. 25 



III. Movements. Observe: 



1. The mode of formation of a pseudopodium. 



2. The process of locomotion. 



3. If possible, the taking in of food and the 



passing out of waste matter. 

 Make a series of outline sketches at regular intervals 

 to show the changes of form. 



4. The effect of higher temperatures on the 



movements of an amoeba. (Heat gradually 

 on a warm stage* Record the result.) 



IV. [Reproduction. 



If an amoeba be found dividing by fission, ob- 

 serve and sketch it. 

 Y. Chemical and Mechanical Tests. 



1. Treat with magenta or iodine. Record the 



result. 



2. Crush a stained specimen. Describe the result. 



* A simple warm stage may be made of sheet copper the width 

 of a glass slide and several times its length, with a hole pierced 

 an inch and a half from one end to correspond to the aperture of 

 the diaphragm. To use the stage, place it on the stand of the mi- 

 croscope and fasten the slide down upon it with the microscope- 

 clips. To heat the copper, place a lighted alcohol-lamp under the 

 free end. A simple method of determining when the temperature 

 of the slide has reached approximately 38 degrees is to place upon 

 it a small fragment of paraffine that has been mixed with suffi- 

 cient benzole to reduce its melting-point to the desired degree. 



