SPERMAPH7TES. 91 



IV. Castor-oil Bean. 



Gently remove the brittle seed- coat, separate the 

 cotyledons, and make very thin sections of 

 the endosperm. 



Mount some in pure glycerine and some in 

 water. Put others into a drop of alcoholic 

 solution of eosin on a slide. After Jive 

 minutes remove the eosin with filter-paper, 

 add 50 per cent, alcohol and a drop of 

 glycerine, and put on a cover-glass. 



1. Study first the glycerine preparation. Ob- 



serve : large aleurone-grains containing 

 spherical bodies (globoids). 



2. Compare the water preparation with the 



glycerine preparation. What difference is 

 observable ? 



Add iodine to the glycerine preparation. 

 What is the effect ? 



3. In the stained preparation look for crystalloids 



in the aleurone-grains. 

 Sketch. 



SEEDLINGS. 

 I. Dicotyledons. 

 A. Observe in the earlier stages the way in which 



the seedling breaks through the ground. 

 Examine and sketch different stages of each of the 

 following forms : 



1. Pea. 



2. Bean. 



