RESPIRA TION. 1 43 



(5) Note the time (one hour or more); clamp siphon 

 tube; turn the stoppers of e and f, clamp x and y; 

 disjoin d and weigh it. 



(6) Weigh e; weigh f; weigh g. 

 Observations. 



1) How much has the animal lost in weight during the 

 period of observation? 



(2) How much water left the animal cage during the 

 period of observation? 



(3) What was the source of this water? 



(4) Did the animal micturate or defecate during the 

 time of the experiment? If so, is this to be looked 

 upon as a source of error in the experiment? Would 

 such an occurrence tend to increase or to decrease the 

 amount of water caught in the CaCl 2 tubes e and f? 

 Would it cause a discrepancy between the loss in 

 weight of the animal, as determined, and the com- 

 bined weight of collected H 2 O and CO 2 ? 



(5) How much CO 2 left the animal cage during the 

 observation ? 



(6) What is the total amount of H 2 O and CO 2 collected? 



(7) Does the amount of these excreta collected equal 

 the loss in weight in the animal? What should the 

 relation of these two quantities be? Explain in full. 



(8) What is the respiratory quotient? 



(9) Formulate several problems which may be solved 

 with this method? 



