RESPIRATION. 145 



and general condition of animal "a" in the normal 

 state, and at the end of every five minutes after the 

 closure of the jar. 



What changes in rate or depth of respiration 

 have been noted? 



(2) Note all abnormal signs and symptoms. 



(3) On post-mortem examination record the condi- 

 tion of heart, large blood vessels, lungs, liver, kid- 

 neys and the general appearance of the tissues. 



(4) Compare the conditions with those found in a 

 normal animal, prepared by the demonstrator. 



Respiration in a larger closed space. 



(5) Note all symptoms of animal " b " every five min- 

 utes after confinement in the jar. 



(6) Make a post-mortem examination; record in de- 

 tail the condition of the organs as in the case of 

 animal " a." 



(7) Compare animal "b" with the normal animal. 



(8) Compare animal " b " with animal " a." 

 Respiration in an atmosphere of one- third CO 2 



(9) Note all symptoms at intervals of five minutes. 



(10) Compare these observations with corresponding 

 ones from animal " a " and animal "b." What are 

 your conclusions ? 



(11) Make a post-mortem examination; make a record 

 as before. 



(12) Compare appearances in animal "c" with those 

 in the normal animal; with those of animal "a;" 

 with those of animal "b." 



(13) Make a generalized statement of the facts dis- 

 covered in the experiments. 



(14) What is the cause of death when an animal is 

 inclosed in a small space? 



