XLI1. Intestinal digestion. 



/. Materials. 2 pig pancreases; 200 c. c. of pig or ox 



bile. 

 2. Preparation. 



(1) Aqueous pancreatic extract (a). 

 (a) Free a pig pancreas of fat. 

 ,(<) Grind it in a meat hasher. 



(V) Extract with water kept at a temperature of 25 

 to 28 C. 



(d) After two hours strain through linen and filter 

 through absorbent cotton. 



( 2 ) Glycerin extract of the pancreatic ferments, 

 (a) After freeing the gland of fat, grind it. 

 (/) Place it in two volumes of absolute alcohol for two 



days. 

 (V) Drain off the alcohol and transfer to 2 volumes of 



pure glycerin. 

 (</) After one week press out the glycerin, which has 



extracted the ferments. 



This glycerin extract will keep indefinitely. To 



make artificial pancreatic juice proceed as follows: 



(e) To 1 volume of the glycerin extract add 5 or 6 

 volumes of water and sufficient sodium carbonate 

 solution to give the mixture a distinctly alkaline 

 reaction. 



(3.) Preliminary experiments on bile. This secretion may 



be easily procured from the slaughter house at almost 



any time in the year, whereas the gastric juice and 



pancreatic juice may only be obtained by resort to 



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