D-IGESTION AND ABSORPTION. 187 



Pancreatic juice contains an amylolytic ferment. 



(2) Subject fibrin to the action of both of the pancre- 

 atic preparations. 



Pancreatic juice contains a proteolytic ferment. 



(3) Boil fresh milk and mix it with an equal bulk of 

 the aqueous extract of pancreas and put the mixture 

 into the incubator. Put also into the incubator 

 boiled milk diluted with an equal volume of distilled 

 water. The milk which is mixed with pancreatic 

 juice will curdle much sooner than the other. 



Pancreatic juice contains a milk curdling ferment. 



(4) Mix 5 or 6 c. c. of neutral olive oil with an equal 

 volume of aqueous extract of pancreas; shake the 

 mixture vigorously. 



No emulsion is formed. Place one-half of the 

 mixture in the incubator. After a few hours any 

 undigested oil may be emulsionized on shaking, or 

 fresh oil may be emulsified. Explain. 



(5) To the second part of the mixture add 3 c. c. bile; 

 shake the mixture vigorously. A good emulsion is 

 formed. How is this emulsion formed? What 

 factor of an emulsion does the bile add ? What is 

 the relation of experiment (5) to experiment (4)? 



Pancreatic juice contains a fat-splitting ferment 

 whose action liberates fatty acids. 



(6) To starch paste add several volumes of dilute 

 bile. Result? 



^7) To fibrin add dilute bile. Result? 



(8) To oil which contains free fatty acid add bile; 

 shake the mixture vigorously; Result? 



(9) To neutral oil add bile; shake the mixture vigor- 

 ously. What is the result? Allow the mixture to 

 stand in the incubator. After several hours shake 

 the mixture. 



