PHYSIOLOGICAL H&MATOLOGY. 



Operation. 



(a) Obtain and spread blood as described in section a. 



(<) Place the cover glass, spread side down upon the 

 heater and maintain at 100C. for fifteen minutes. This 

 process dries and fixes the preparation. 



(V) Remove the fixed preparation; cover the film with 

 staining solution, allowing it to remain from six to ten 

 minutes. The time of staining depends upon the 

 length of time the film has been heated; a film fixed 

 quickly will stain more readily. 



FIG. 51. 

 FIG. 51. Plan for constructing the water fixing plate. 



(//) Rinse off the excess of stain in pure water and dry. 

 (<?) Mount in balsam. 



4. Precautions. Be sure that the water in the heater is 

 boiling before placing the films upon it. Do not let 

 the water boil too violently or it may boil over and 

 spoil the films. The films must be air dried before 

 they are placed upon the heater. 



