LXVI. Pilocarpin. 



/. Material. 



1 rabbit; 1 dog; hydrochlorate of pilocarpin; sulphate of 

 morphin; sulphate of atropin; chloroform. 



2. Preparation. 



Make solution of pilocarpin, 50 mgrms. to 10 c.c.; atro- 

 pin, 0.02 grm. to 10 c.c. ; morphin 0.6 to 10 c.c. 



Do not fasten the rabbit to the holder. Fasten the 

 dog to the dog board, after giving preliminary hypoder- 

 mic injection of 0.03 grms. morphin. 



j. Experiments and Observations. 



(1) Give, hypodermically, 0.02 grm. pilocarpin to 

 the rabbit. 



(a) Record symptoms as they arise, especially as 

 regards: 



(I) Secretions; 



(II) Pulse rate; 



(III) Size of pupil; 



(IV) Temperature. 



() Formulate the total effect of pilocarpin upon the 

 animal. 



(2) After morphinizing the dog, fasten it firmly to the 

 dog-board and lightly anaesthetize; expose both vagi. 



Count the pulse. Give a subcutaneous injection of 

 0.03 grms. pilocarpin. After salivation has become 

 profuse count the pulse again. 



How does pilocarpin affect the pulse rate ? 



(3) Now sever the vagi. 



(a) How does the severing of the vagi affect the nor- 

 mal animal? (See page 109. ) 



