MUSCULAR TISSUE. 95 



around the blood-vessels ; and again running in 

 various directions and associated with bands of con- 

 nective tissue, they form large compact masses, as in 

 the uterus. 



The longitudinal striation which, under favorable 

 circumstances, is seen on the cell-body is not a 

 mere surface marking, but extends deep into the 

 cell, as may be seen in transverse sections of suitably 

 prepared cells where fine lines are observed passing 

 inward from the periphery of the cell toward the 

 nucleus. The blood-vessels supplying this tissue 

 form for the most part elongated net-works through- 

 out its substance,, 



TECHNIQUE. 



Isolated Cells. These we obtain by teasing bits of the 

 tissue, but as they are firmly bound together by the cement- 

 ing substance, this must first be dissolved or softened. 

 This can be conveniently accomplished by soaking a bit 

 of the tissue the wall of the intestine, for example, in 

 a forty-per-cent. aqueous solution of potasic hydrate for 

 fifteen minutes ; it is then transferred to a large quantity 

 of a sixty-per-cent. solution of potassium acetate contain- 

 ing one-per-cent. of hydric acetate. This checks the 

 action of the potasic hydrate. It is then transferred to a 

 saturated aqueous solution of potash alum for twenty-four 

 hours, then stained in alum carmine, teased apart, and 

 mounted in a forty-per-cent. aqueous solution of glycerin. 



Transverse and Longitudinal Sections of the Cells. 

 The intestine of the cat is well adapted for this prepara- 



