CIRCULATION OF THE BLOOD. 



-F- 



other with defibrinated rabbit's or dog's blood diluted with equal 

 volumes of o.6-per-cent NaCl solution. 



Perfuse with the physiological salt solution. From time to time, 

 close the outlet tube of the manometer and record the changes in 

 ventricular pressure on a revolving drum of medium speed. Con- 

 tinue the perfusion until 

 the rhythmical beat 

 ceases. 



Now perfuse with the 

 diluted defibrinated 

 blood. Do the rhyth- 

 mical pulsations again 

 begin ? 



Repeat with a fresh 

 heart preparation, using 

 the diluted blood from 

 the start. How long 

 are rhythmical contrac- 

 tions continued with the 

 blood mixture as com- 

 pared with the NaCl so- 

 lution alone ? Record 

 the pressure changes as 

 before. 



Fill one perfusion tube 

 with physiological saline, 

 saturated with ether. 

 Perfuse the heart with 

 this solution. Record 

 the pressure changes 

 with the manometer. 



Compare the effect of ether perfusion with that obtained through 

 the direct application of the drug. 



Change the perfusion to diluted blood, thoroughly washing out 

 the ether solution. Do the heart-beats return to the normal? 



[93l 



FIG. 29. Frog's Heart Perfusion Apparatus. 

 (Kronecker.) //, Heart ; C, cannula ; G, glass 

 containing physiological salt solution; E, wires 

 of constant current ; F, perfusion flasks ; Af, 

 manometer. 



