LABORATORY MANUAL OF PHYSIOLOGY. 



Clip and shave the hair over the * midabdominal region and 

 wash the skin with corrosive sublimate, i to 1000, followed by al- 

 cohol. Clean the hands by thorough scrubbing and immersion in 

 the corrosive-sublimate solution. 



Open the abdomen by an incision in the median line. Locate 

 the head of the pancreas at the duodenum and the tail at the spleen. 

 Carefully separate the pancreas from the omentum and mesentery 

 and from the duodenum, tying the pancreatic ducts and all ar- 

 teries and veins with double ligatures and cutting between. 

 Having controlled hemorrhage in this way, remove the pancreas 

 in tola. 



Bring the peritoneum and abdominal muscles together with one 

 row of interrupted sutures and close the skin wound with a second 

 row of sutures. Dry the wound with alcohol and ether and paint 

 with collodion. 



After the animal has recovered from the anaesthetic and the im- 

 mediate shock of the operation, collect the urine every six hours and 

 make quantitative estimations of the sugar by Fehling's method. 

 Also, carefully note the condition of the dog, as to loss of weight, 

 temperature, appetite, weakness, convulsions, etc. The diet may 

 be the same as that given previous to the operation. Make an es- 

 timate of the carbohydrates, proteids, and fat of the diet. 



If the dog survives more than forty-eight hours after the oper- 

 ation, the diet may be changed to pure proteid and a known 

 amount of raw pancreas given with the food. 



Immediately after the death of the animal, the liver should be 

 removed and tested for glycogen as described for experiment I. 



III. THYROID. 



1. Ablation of the Thyroid in Dogs. This is best done in 

 two operations. At the first operation one lobe of the thyroid is 

 removed, and at the second the other lobe. 



Select a young dog. Record weight. Place under morphine 

 narcosis. Shave and scrub skin of the neck and wash with a bi- 

 chloride solution, i to icoo. The operation should, of course, be 



