LABORATORY MANUAL OF PHYSIOLOGY. 



2. Thyroid Feeding after Thyroid Removal. Remove the 

 thyroid of another dog in the same way as in experiment i , and 

 at about the same time, so that the symptoms of the two animals 

 may be compared. The second dog should be fed, after the com- 

 plete removal of the gland, with fresh sheep's thyroids, or thyroid 

 extracts may be mixed with the food. 



What are the symptoms of thyroid removal in the dog ? How d< 

 they compare with those stated to occur in similar conditions in 

 man ? How do they compare with the symptoms of thyroid dis- 

 ease in man? Has thyroid feeding any effect in alleviating the 

 symptoms following thyroid removal in the dog ? 



If the dog fed with thyroids survives, it should be killed later 

 and careful search made for accessory bodies. 



IV. SUPRARENAL GLANDS. 



1. Ablation of the Suprarenal in the Rabbit. Demonstra- 

 tion. Select a large, well-nourished rabbit. The Belgian hare 

 serves well for the purpose. Starve for twenty-four hours. The 

 operation is done in two stages, one suprarenal being removed at 

 the first operation, and the other two weeks later. The left 

 suprarenal is removed most readily by the abdominal route. The 

 right suprarenal is reached best by the dorsal route without enter- 

 ing the peritoneal cavity. 



Inject under the skin 9 cgm. of morphine sulphate. Strap the 

 rabbit, back down, upon the rabbit-board. Clip and shave the hair 

 from the midabdominal region. Wash with bichlorid. Sterilize 

 instruments by boiling. Sterilize absorbent cotton pads in o.8-per- 

 cent NaCl solution. 



Beginning just below the xyphoid appendix of the sternum, 

 make an incision in the midabdominal line, through the skin, 

 fascia, and peritoneum. The length of the incision should be 

 about three inches. Cover the intestines and hold them back with 

 absorbent cotton pads wrung out in hot salt solution. Let an as- 

 sistant hold back the edges of the abdominal wound with retrac- 

 tors. Locate the left kidney. Follow the renal vein to its junction 



