LABORATORY MANUAL OF PHYSIOLOGY. 



nerves. Compare the effect of the second injection upon heart- 

 beat and blood pressure with the result of the first injection, when 

 the vagus nerves were intact. 



(c) Repeat, using in place of the extract of the rabbit's 

 suprarenal, i c.c. of a i to 10,000 solution of adrenalin 

 chlorid. 



The effect of the suprarenal extract upon skeletal muscle has 

 already been shown under Muscle-nerve Physiology. 



4. Remove the second suprarenal from rabbit i as follows: 

 Place the animal under morphine and ether. Tie, belly down, 

 upon the operating-board, placing a pad under the abdomen for 

 the purpose of bringing the viscera into the field of the operation. 

 Shave and cleanse the skin of the right flank. Beginning at the 

 lower border of the ribs, make a longitudinal incision through the 

 skin and fascia of the flank about three inches long and about two 

 inches from the spinous processes of the vertebrae. Cut through 

 the lumbar aponeurosis of the abdominal muscles, and separate 

 these from the heavy spinous muscles. Locate the kidney. Let 

 an assistant, standing on the right side of the operating-table, hold 

 the kidney down and to the right with one finger, and with two fin- 

 gers of the other hand pull the lateral margin of the wound up 

 and out. The operator should stand to the left of the subject. 

 Follow up the renal vein as before, until the right suprarenal body 

 is seen. Separate this from its attachments in the same way as 

 was done in removing the left suprarenal. Remove the gland en- 

 tire, or, if this cannot be done with safety, remove as much as pos- 

 sible and crush the remainder with forceps. Sew up the wound 

 with two rows of sutures and return the rabbit to its cage. Since 

 death frequently occurs within the first twelve hours following com- 

 plete removal of the suprarenals, the operation is preferably done 

 in the morning, so that the animal may be under observation all 

 day. If the operation has been successfully performed with the 

 minimum of shock and hemorrhage, the animal should regain con- 

 sciousness. Make careful note of all symptoms from the time of 

 the operation until death occurs. 



