EXCRETION. 



(c) Allow the blood pressure to return to normal. Allow the 

 animal to inhale a few. whiffs of amyl nitrite. Note the effect 

 upon blood pressure, kidney volume, and urine flow. 



(d) After the blood pressure has again returned to normal, in- 

 ject into the jugular vein one cubic centimetre of a i to 10,000 

 solution of adrenalin chlorid. What is the effect upon the blood 

 pressure, kidney volume, and urine flow ? 



(e) After the blood pressure has returned to normal, connect 

 the vein cannula with a burette containing warm physiological salt 

 solution. Being careful that there are no air bubbles in the con- 

 necting tubes, allow the solution, under low pressure, to run slowly 

 into the vein. 



Run in fifty cubic centimetres of the solution. Is there any 

 noticeable rise of blood pressure ? Explain. Is there any change 

 in kidney volume or urine flow ? 



(/) Run into the vein fifty cubic centimetres more of the solu- 

 tion. Note any effect upon blood pressure, kidney volume, or 

 urine flow. 



(g) Repeat the perfusion, using fifteen cubic centimetres of a 

 i-per-cent urea solution. 



4. Intravenous Injection of Dextrose. Using the same rab- 

 bit as in the previous experiment, or a fresh animal if necessary, 

 prepare a i -per- cent dextrose solution. Warm this to body 

 temperature and slowly inject twenty cubic centimetres of this 

 solution into a vein. Collect the urine eliminated before and 

 after the sugar injection. Test both with Fehling's solution for 

 reducing substances. Collect samples of the urine every ten 

 minutes after the beginning of the sugar injection. When does 

 the sugar first appear in the urine ? When does it cease to ap- 

 pear in the urine ? 



5. Intravenous Injection of Albumin. Test the urine for al- 

 bumin. If there is none present, inject, into a vein, ten to fifteen 

 cubic centimetres of a i-per-cent solution of egg albumin in physio- 

 logical salt solution. Examine the urine for albumin at intervals 

 of ten minutes. 



[169] 



