LABORATORY MANUAL OF PHYSIOLOGY. 



6. Intravenous Injection of Peptone. After the disappear- 

 ance of the albumin from the urine, prepare a 2-per-cent peptone 

 solution. Inject 10 c.c. of this solution into the vein. Collect 

 the urine for ten minutes or until enough has been eliminated for 

 testing. 



Saturate the urine with ammonium sulphate. This precipitates 

 mucin, albumin, and urates. Filter and test the nitrate for pep- 

 tones by means of the biuret reaction. 



Do peptones occur, normally, in the blood stream ? If not, what 

 becomes of the peptones that are absorbed from the gastric and 

 intestinal mucous membranes ? Is peptone ever found as an ab- 

 normal constituent of the urine ? Under what pathological con- 

 ditions may peptonuria occur? 



7. Effect of Peptone on the Coagulation of the Blood. Iso- 

 late and introduce a cannula into one carotid artery. Open the 

 clamp on the artery and collect 10 or 15 c.c. of blood in a small 

 test tube. Note the time taken for solidification of the shed 

 blood. 



Now inject 15 to 20 c.c. of the peptone solution into the vein. 

 In three or four minutes open the artery clamp again ; allow 2 or 

 3 c.c. to escape, and then collect in a small test tube 10 to 15 c.c. 

 of blood. Compare the coagulability of this second sample with 

 that of the first portion of blood shed. What is the effect of pep- 

 tone upon the coagulability of the blood? 



