STAINING OF BACTERIA. 33 



Y. Alkaline Methylin Blue. 



A. Lqffler's. 



Sat. ale. Sol. methylin blue . . 30 

 Sol. potass, hydrat. (1-10,000) . 100 M. 



B. Koch's. 



Sol. potass, hydrat. (10 per cent.) 0.2 



Sat. ale. sol. methyl, blue . . 1.0 

 Distilled water .... 200.0 M. 



VI. Carbolic Acid Solutions. 



A. Ziehl-Neelsen. 



Fuchsin (powd.) .... 1 part. 



Alcohol 10 parts. 



5 per cent. sol. acid, carbolic . 100 parts. M. 

 Filter. The older the solution the better. 



B. Kuhne. 



Methylin blue . . . . 1.5 



Alcohol 10.0 



5 per cent. sol. ac. carbol. . . 100.0 

 Add the acid gradually. This solution loses strength with age. 



VII. Gram's lodin Solution. 



Iodine 1 



Potass, iod 2 



AquaB destillat 300. M. 



VIII. -Loner's Mordant (for flagella). 

 Aq. sol. of tannin (20 per cent.) . 10 parts. 

 Aq. sol. ferri sulph. (5 per cent.) . 1 part. 

 Aqure decoc. of logwood (1-8) . 4 parts. M. 

 Keep in well-corked bottle. 



IX. Unna's Borax Methyl Blue. 



Borax 1 part. 



Methyl blue 1 part. 



Water 100 parts. M. 



