34 ESSENTIALS OF BACTERIOLOGY. 



X. Gobbet's Acid Blue (rapid stain). 

 Methylin blue .... 2 

 25 per cent, sulphuric acid . . 100. M. 



XI. Alkaline Anilin Water Solutions. 

 Sodium hydrat. (1 per cent.) . . 1 

 Anilin oil water .... 100. M. 

 And add 



Fuchsin, or methyl-violet powd. . 4 

 Cork well. Filter before using. 



XII. Roux's Double Stain. 

 Dahlia or gentian violet ... 0.5 gramme. 



Methyl green 1.5 " 



Distilled water 200.0 grammes. M. 



Use as other stains, without add. 



XIILNtisser's Stain. (For Diphtheria.) 



Solution I. 



Methylin blue 1 gramme. 



Alcohol (96 per cent.) . . .20 c.c. 

 Dissolve and add water . . . 950 c.c. 

 Glacial acetic acid .... 50 c.c. M. 



Solution II. 

 Vesuvin ...... 2 grammes. 



Water 1000 c.c. M. 



Stain cover-glasses (1) three seconds in Sol. I. ; (2) wash in 

 water; (3) three seconds in No. 2; (4) wash in water. Body of 

 bacillus, brown ; oval granules at each end, blue. 



XlV.Carbol-thionin. (Mcolle.) 



Sat. sol. thionin in ale. (90 per cent.) 10 c.c. 

 Aqueous sol. ac. carbol. (1 per cent.) 100 c.c. M. 

 Stain sections, one-half to one minute. 



