46 



ESSENTtALS OP BACTERIOLOGY. 



The autoclave of Chamber-land allows a temperature of 120 

 C. to be obtained, and is much used in Pasteur's laboratory. 



FIG. 17. 



Chamberland's Autoclave with pressure. 



Instead of sterilizing for a long time at once, successive steri- 

 lization is practised with nutrient media, so that the albumen 

 will not be too strongly coagulated. Fifteen minutes each day 

 for three days in succession. 



