52 ESSENTIALS OF BACTERIOLOGY. 



FlG. 23. 



Method of inoculation. (Woodhead and Hare.) 



Bread Mush. Bread devoid of crust, dried in an oven, and 

 then pulverized and mixed with water until thick and sterilized 



i 



as above. 



CHAPTER IX. 



SOLID TRANSPARENT MEDIA. 



Solid Transparent Media are materials which can be used for 

 microscopical purposes and which can readily be converted 

 into liquids. Such are the gelatine and agar culture media. 



Gelatine. Gelatine is obtained from bones and tendons, and 

 consists chiefly of chondrin and gluten. 



The French golden medal brand is the one most in use, found 

 in long leaves with ribbed lines crossing them. 



Koch-Loffler 10 per cent. Bouillon-Gelatine. To the meat- 

 water as made for the bouillon are added 

 100 grammes gelatine, 

 10 " peptone, 



5 " salt, 



to each 1000 grammes of the meat-water; or to the bouillon 

 made from beef-extract the gelatine is added ; this is placed in 

 a flask and gently heated until the gelatine is dissolved. 



Neutralization with the soda and thon cooking in water-bath 

 or carefully boiled over flame for 1 hour or more until the 



