,ANSPA 



Many combinations of the preceding are also in use, such as 

 glucose-agar, glucose gelatin, blood- or serum-agar ; and litmus 

 is often added to media to show changes in reaction during 

 bacterial growth. 



Dunham's Rosalie Acid Solution. 



Peptone sol. (Dunham) .... 100 c.c. 

 2 per cent. sol. rosalic acid . . .0.5 gr. 

 Alcohol (80 per cent.) . . . .100 c.c. 

 M. To detect acids and alkalies. 



Eisner's Medium (for typhoid). (lodo- potass. Potato-gelatin.) 

 Five hundred grammes of (peeled and washed) potatoes are 

 mashed and pressed through a fine cloth. The juice is allowed 

 to settle, is filtered, and after 1 hour's cooking has added to it 10 

 per cent, gelatin; then 2? c.c. ^ normal sodic hydrate solu- 

 tion, and finally 1 per cent, potassic iodid. 



Typhoid Medium of Hiss. This consists of a slightly acid 

 mixture of gelatin and agar, beef-extract, sodium chloride, and 

 dextrose, used in different proportions for plate and tube cul- 

 tures. It is semi-solid in character and facilitates the identi- 

 fication of the motile typhoid bacilli which produce a uniform 

 clouding through the medium in tubes. 



Urine Media (Gonococci). 



Urine (sterile taken) . . . , .1 part. 

 2 per cent, agar solution . . . . 1 " 



Fresh Egg Cultures, after Hiippe. The eggs in the shell are 

 carefully cleaned, washed with sublimate, and dried with cotton. 



The inoculation occurs through a very fine opening made in 

 the shell with a hot platinum needle ; after inoculation, the open- 

 ing is covered with n piece of sterilized paper, and collodion. 



Boiled Eggs. Eggs boiled, shell removed over small portion, 

 and the coagulated albumen stroked with the material. 



Guinea-pig Bouillon. The flesh of guinea-pigs as well as that 

 of other experiment-animals is used instead of beef in the prep- 

 aration of bouillon, for the growth of special germs. 



The extracts of different organs have been added to the 

 various media for experimentation. 



