60 



ESSENTIALS OF BACTERIOLOGY, 



CHAPTER X. 



INOCULATION OF GELATINE AND AGAR. 



Glass Slide Cultures. Formerly the gelatine was poured on 

 little glass slides such as are used for microscopical purposes, 

 and after it had become hard, inoculated in separate spots as 

 with potatoes. 



FIG. 29. 



Manner of holding tubes for inoculation : a, tube with material ; b, tube to be 

 inoculated ; c, cotton plugs. (After Woodhead and Hare.) 



Test Tube Cultures. The gelatine, agar, or blood serum having 

 solidified in an oblique position, is smeared on the surface with 



