108 ESSENTIALS OF BACTERIOLOGY. 



nodules contain the bacilli in large numbers. By applying a 

 vesicant to the leprous skin the serum thereby obtained will con- 

 tain great numbers of bacilli. This is a simple diagnostic test. 



Method of Infection. Not yet determined ; the air, soil, water, 

 and food of leprous districts have been carefully examined with- 

 out result. The nasal secretion is very infectious. 



Syphilis Bacillus of Lustgarten (Smegma Bacillus of Alvarey 

 and Tavel). Lustgarten in 1885, through a certain staining 

 process, found peculiar bacilli in syphilitic tissues which he 

 thought had a direct connection with the disease. 



Van Niessen, Joseph and Piorkowski, DeLisle and Jullien all 

 describe other organisms that they have found in syphilitic 

 lesions, and Schiiller mentions a protozoon-like body he has 

 seen in many cases, but these results still lack confirmation. 

 Metschnikoff, Roux, and Lassar have lately succeeded in inocu- 

 lating chimpanzees with what appears to be true syphilis. 



The question yet remains an open one, what relation the 

 syphilis or the smegrna bacillus bears to syphilis, and will 

 remain so until the bacillus can be cultivated, which so far has 

 not been accomplished. 



Bacillus of Glanders. (Bacillus Mallei, Loffler-Shutz.) Botz 

 bacillus. 



Origin. In the " farcy buds" or little nodules of the disease, 

 by Loffler and Shiitz in 1882. 



-Form. Small slender rods, about the size of the tubercle 

 bacillus. The ends rounded. Never appearing in large collec- 

 tions, usually singly. Spores are said to exist, but this is 

 doubtful. 



Properties. The rods are very resistant, living in a dried state 

 for three months and longer without any spores present. They 

 are not motile ; possess, however, great molecular vibration. 



Growth. The growth occurs between 25 and 40 C., best 

 at 37 C. ; it is very sparse upon gelatine, but on glycerine-agar 

 or blood serum a very abundant growth occurs. 



Colonies. On agar or glycerine-agar there appear in two to 

 three days small white glistening drops, which under microscope 

 seem as round granular masses with an even periphery. 



Stroke Cultures. On glycerine-agar and blood serum small 



