PATHOGENIC BACTERIA. 



109 



transparent drops of whitish or grayish color, which soon 

 coalesce to form a broad band. 



Potato. An amber-colored honey-like growth which gradually 

 turns red, then brown, and greenish-brown around it. Weakly 

 acid potatoes are a good medium and give the most typical 

 growth. 



FIG. 55. 





m > 



" 



Bacillus of Glanders. 



Staining. Since the bacillus is very easily decolorized, some 

 special methods have been recommended. 

 L6ffler*s. (For cover-glass preparations.) 



1. Alkaline methylin blue (Loffler's). 5 minutes. 



2. Acetic acid with a few drops of tropseolin. 1 second. 



3. Washed in water. 



For Sections. Instead of tropseolin acetic acid, the following 

 mixture is used : 



$. Oxalic acid 5 per cent. . . . gtt. j. 



Cone, sulphuric, acid gtt. ij. 



Aq. destill. ij. M. 



sections are kept in this 5 seconds. 

 Kukne^s method. Coverglass. 



1. Warm carbol-blue 2 min. 



2. Decolorized in weak sol. of muriatic acid (10 parts to 500). 



3. Washed in water. 



