132 



ESSENTIALS OF BACTERIOLOGY. 



PIG. 69. 



Stab Culture. A growth along the needle-track, but on the 

 surface a button-like projection, which gives to the growth the 

 appearance of a nail driven into the gelatine, 

 its head resting on the surface ; therefore 

 such cultures are called "Nail cultures." 

 See Fig. 69. Old cultures are colored brown, 

 and contain bubbles of gas. 



Potato. A yellow, moist layer in a few 

 days at brood-heat. Gas bubbles develop. 



Staining. The ordinary aniline stains. 

 The sections do not take Gram's method ; 

 are therefore not suited for double staining. 



Capsule. Stained as follows : 



Cover glasses. 



1. Acetic acid, two minutes. 



2. Allow acetic acid to dry by blowing air 

 upon it through a glass tube. 



3. Saturated, aniline water. Gent, violet, 

 ten seconds. 



4. Rinse in water. Mount in Canada balsam. 

 For Sections. 



Bacillus of Pneumo- f cone. ale. gent, violet, 50.0 



nia. stab Culture. 1. Stain in warm 1 aqua, 100.0 



<**"*> (acetic acid, 10. 



M. for 24 hours. 



2. Rinse in one per cent, acetic acid. 



3. Alcohol to dehydrate. Mount in balsam. 



The capsule will be found stained a light blue, the bacillus a 

 deep blue. (See also the capsule stain of Hiss, p. 35.) 



Pathogenesis. Animals are not affected unless the culture is 

 injected intrapleura. 



Pneumobacillus of Frankel. (A. Frankel and Weichselbaum.) 



Synonyms. Pneumococcus ; Diplococcus of Pneumonia ; Mi- 

 crococcus of sputum septicaemia ; Micrococcus Pasteuri ; l)iplo- 

 coccus lanceolatus. 



Origin. A. Frankel found it in the sputum of pneumonic 



