134 ESSENTIALS OF BACTERIOLOGY. 



Bouillon. They grow better here than in the other media, 

 remaining alive a longer period of time. 



Staining. Takes Gram's method and the other aniline stains 

 very readily. The capsule stained the same way as that of the 

 Friedlcinde-r bacillus. 



Pathoyenesis. Rabbits and guinea-pigs, if subcutaneously in- 

 jected, die in the course of a couple of days with septicaemia. 

 (0.1 c.cm. of a fresh bouillon culture suffices.) 



Autopsy shows greatly enlarged spleen and myriads of bacilli 

 in the blood and viscera, the lungs not especially affected. If 

 injected per trachea, a pneumonia occurs. In man in 90 per 

 cent, of croupous pneumonia they are found and usually only 

 during the existence of the rusty sputum, i. e., the first stage. 



FIG. 71. 



Micrococcus tetragenus in sputum (tubercle bacillus also). 



They have also been found in pleuritis, peritonitis, pericarditis, 

 meningitis, and endocarditis. They stand in some intimate re- 

 lation with all infectious inflammations of the body. Their 

 presence in healthy mouth secretion does not speak against 

 this, it requiring some slight injury to allow this ever-present 

 germ to develop into disease. 



Anti-toxin of Pneumonia. (Klemperer.) 



The injection of very diluted cultures of the virulent bacilli in- 

 travenously has produced an immunity in rabbits and guinea- 

 pigs. The serum of such artificially immune animals when filtered 



