144 ESSENTIALS OF BACTERIOLOGY. 



83 and 37 C., and the growth occurs very slowly and 

 sparsely. 



Method of Cultivation (Wertheim). Gonorrhoea! pus is mixed 

 in a test-tube with liquid human blood serum of 40 C. temper- 

 ature, and two dilutions are made with blood of the same tem- 

 perature. An equal quantity of 2 per cent, agar solution is 

 now poured into each tube, and three glass dishes are covered 

 at once with this mixture. After being in the brood oven for 

 twenty-four hours colonies can be discovered. 



FIG. 80. 



Gonococcus in urethral pus ; X 1000 (Frankel and Pfeiffer). 



In three days a very thin, almost invisible, moist j^ellowish 

 growth, seemingly composed of little drops. 



Under low power small processes are seen shooting out from 

 the smooth border. 



It requires to be then transferred to fresh media, as it quickly 

 perishes. 



Cultivation has also occurred on acid gelatine, gelatine con- 

 taining acid urine, in acid urine itself, and albuminous urine 

 with agar. 



Staining. Colored easily with all ordinary aniline stains. 



