178 APPENDIX. 



of the ovoid or club-shaped hyphae lie detached from the 

 clusters. Through cultivation it was found that the ovules give 

 rise to filaments, and they then form the ovules again. 



Cultivation. At 38 C. on glycerine-agar in a period of one 

 to two weeks, pointed scales about the size of a millet-seed, 

 center dry and prominent, margins hyaline, composed only of 

 filaments, short and long, massed together, but no clubbed 

 forms. 



By some the clubs are considered the spore organs ; by others 

 they are thought to be encapsulated or thickened filaments. 



Pathogenesis. When a portion of the growth obtained in 

 eggs was injected into the abdominal cavity of a rabbit, actinomy- 

 cotic processes developed upon the peritoneum. 



It usually gains access to the living body through a wound in 

 the gum or some caries of the teeth. A new growth is formed, 

 ulceration being first set up. 



The new tissue, composed of round cells, then undergoes soft- 

 ening, purulent collections form and the normal structure i? 

 destroyed. 



The usual seat is in the maxillary bones, but the fungus has 

 been found in the lungs, tonsils, intestines, and various other 

 organs in man and cattle. 



Examination. Well seen in the unstained condition. From 

 the pus or scraping a small portion is taken and squeezed upon 

 the glass slide ; if calcareous matter is present, a drop of nitric 

 acid will dissolve the same. 



Glycerine will preserve the preparation. 



Staining. Cover-glass specimens stained best with Gram's 

 method. Tissue sections should be stained as follows : 



Ziehl's carbol-fuchsin, ten minutes. Rinse in water. 



Cone, alcohol. sol. of picric acid, five minutes. Rinse in water. 



Alcohol, 50 per cent., fifteen minutes. Alcohol absolute, clove 

 oil, balsam. 



The rays stained red, the tissue yellow. 



Streptothrix Madura. (Vincent.) 



Origin. Found in the disease known as Madura foot, or 

 Mycetoma, an ulceration affecting the feet, especially of indi- 



