5O ESSENTIALS OF BACTERIOLOGY 



II. Weak Solutions 



Made by adding about i part of stock solution (I) to 10 

 parts of distilled water. This is the ordinary solution in use. 



III. Anilin-oil Water 



Anilin-oil 5 parts 



Distilled water 100 " M. 



Shake well and filter. To be made fresh each time. 



IV. Anilin-oil Water Dyes 

 Saturated alcoholic solution of the 



dye ii parts 



Anilin-oil water 100 " 



Absolute alcohol 10 " M. 



Can be kept ten days. 



V. Alkaline Methylene-blue 



A. Loffler's: 



Saturated alcoholic solution methy- 

 lene-blue 30 parts 



Solution potassium hydroxid (i per 



cent.) i part 



Water q. s. 100 parts M. 



B. Koch's: 



Solution potassium hydroxid (10 per 

 cent.) 2 parts 



Saturated alcoholic solution methy- 

 lene-blue 10 " 



Distilled water 2000 " M. 



VI. Phenol Solutions 



A. Ziehl-Neelsen: 



Fuchsin (powdered) i part 



Alcohol 10 parts 



5 per cent, solution phenol 100 " M. 



Filter. The older the solution, the better. 



