78 ESSENTIALS OF BACTERIOLOGY 



Boiled Eggs. Eggs boiled, shell removed over small por- 

 tion, and the coagulated albumen stroked with the material. 



Guinea-pig Bouillon. The flesh of guinea-pigs, as well 

 as that of other experiment animals, is used instead of beef 

 in the preparation of bouillon, for the growth of special germs. 



The extracts of different organs have been added to the 

 various media for experimentation. 



Wertheim's Medium for Gonococcus: 



Nutrient agar 2 parts 



Human blood-serum or hydrocele 



fluid i part 



Melt agar and cool to 45 C.; then add serum. Tube on 

 slant or pour in Petri plate. Glycerin or glucose can be added 

 to enrich. 



Solution Dried Blood Albumin (King) : 



Blood albumin (commercial) 15 parts 



Glucose bouillon 85 



Dissolve, tube, inspissate, and sterilize as for blood-serum. 



CHAPTER XI 



INOCULATION OF CULTURE-MEDIA 



Glass Slide Cultures. Formerly the gelatin was poured 

 on little glass slides, such as are used for microscopic purposes, 

 and after it had become hard, inoculated in separate spots as 

 with potatoes. 



Test-tube Cultures. The gelatin, agar, or blood-serum 

 having solidified in an oblique position is smeared on the 

 surface with the material, and the growth occurs along the 

 smear, or the medium is punctured with a stab of the plati- 

 num rod containing the material, and the growth follows 

 the line of thrust. The former is called a stroke or smear 

 culture, the latter a stab or thrust culture. 



