82 



ESSENTIALS OF BACTERIOLOGY 



CHAPTER XII 



CULTIVATION OF ANAEROBIC BACTERIA 



SPECIAL methods are necessary for the culture of the ana- 

 erobic variety of bacteria in order to procure a space devoid 

 of oxygen. 



Liborius's High Cultures. The tube is rilled about 

 three-quarters full with gelatin, which is then steamed in a 

 water-bath and allowed to cool to 40 C., when it is inoculated 



Fig. 26. Liborius's method. 



Fig. 27. Hesse's method of making 

 anaerobic cultures (McFarland) . 



by means of a long platinum rod with small loop, the move- 

 ment being a rotary vertical one, and the rod going to the 

 bottom of the tube. 



The gelatin is next quickly solidified under ice; very little 

 air is present. The anaerobic germs will grow from the 



