THE MICRO-ORGANISM OF SYPHILIS AND ALLIED ORGANISMS 213 



which shows no hemolysis after twenty-four hours contains 

 i' unit of antigen provided twice that amount will not prevent 

 hemolysis when no serum is added. 



Having found out the exact amount of guinea-pig serum 

 (complement) necessary to unite with hemolytic amboceptor 

 (rabbit serum) in order to hemolyze blood-corpuscles, this 

 amount is mixed with syphilitic antigen plus the suspected 

 syphilitic serum amboceptor, and incubated for one hour at 

 37 C. // the amboceptor is syphilitic, it will combine with 

 the antigen and guinea-pig complement. To find out if the 

 complement has been bound, the hemolytic amboceptor and 

 its antigen sheep corpuscles are added to the mixture, 

 and if no hemolysis takes place, the complement is fixed and the 

 patient's serum contains the syphilitic antibodies or amboceptors. 



To Set Up Test. Nine tubes needed for Wassermann reac- 

 tion and control. Into each tube i c.c. diluted complement 

 guinea-pig serum. Into tubes 1,2, and 9, 0.2 c.c. of patient's 

 serum. Into tubes 3 and 4, control, syphilitic serum 0.2 c.c.; 

 in 5 and 6, normal serum as control, 0.2 c.c. ; antigen extract, 

 i unit placed in 1,3, 5, and 7. 



To each tube is now added sufficient normal salt solution 

 to make 3 c.c. Tubes gently shaken and placed in incubator 

 at 37 C. one hour. At end of the hour to each tube is added 



1 unit of suspension sheep corpuscles, and to all but No. 9 



2 units of standard amboceptor, in i c.c. saline. 



The tubes again placed in incubator for one hour, readings 

 taken, and then placed in ice-box twenty-four hours, when 

 final results noted. If Wassermann positive 



No. i. No hemolysis. 



No. 2. Complete hemolysis. 



No. 3. No hemolysis. 



No. 4. Complete. 



No. 5. Complete. 



No. 6. Complete. 



No. 7. Complete. 



No. 8. Complete. 



No. 9. No hemolysis. 



