THE MICRO-ORGANISM OF SYPHILIS AND ALLIED ORGANISMS 21$ 



taining 0.4 c.c. of the antigen emulsion, o.i c.c. of 10 per 

 cent, suspension of corpuscles, and 0.6 c.c. of salt solution 

 should show no hemolysis after two hours at 37 C. 



(2) Anticomplementary Bodies. A tube containing 0.4 c.c. 

 of antigen, o.i c.c. of a 40 per cent, dilution of complement, 

 2 units of amboceptor, and 0.6 c.c. of salt solution is incu- 

 bated for one hour and o.i c.c. of 10 per cent, corpuscle sus- 

 pension added. In two hours there should be complete 

 hemolysis. 



(3) Antigenic Properties. After incubating for one hour a 

 tube containing 0.02 c.c. antigen, 0.02 c.c. of a known syphilitic 

 serum, o.i c.c. of a 40 per cent, dilution of complement, 2 

 units of amboceptor, and 0.8 c.c. of salt solution, o.i c.c. of a 

 10 per cent, corpuscle suspension is added, and the tube 

 returned to the incubater. At the end of two hours there 

 should be no hemolysis. 



(4) Amboceptor and complement are titrated the same as 

 in the Wassermann reaction, except that a i per cent, sus- 

 pension of human corpuscles and 0.02 c.c. of complement 

 and antihuman amboceptor are used. 



NOGUCHI SCHEME 



