(b) Total Sulphur and Phosphorus: Sulphur was determined by the 

 method adopted by the the Association of Official Agricultural Chem- 

 ists (63). The filtrate from the barium chloride precipitation was used 

 to determine phosphorus (64). 



(c) Ash: Inorganic matter was estimated by direct incineration of 

 the dried substance in a platinum crucible over a very low flame. 



(d) Moisture: The medium was cooled slowly to 43 C. The agar for 

 the entire series of determinations was obtained from the same flask 

 and the necessary number of petri dishes filled at the same time. The 

 organisms were grown and collected as described above. About o.i gram 

 of growth was rapidly transferred to a rubber stoppered glass vial weigh- 

 ing about 0.3 gram. Two sets of determinations of water content were 

 made: (i) by drying in vacuo (o-iomm. Hgand 22 C.) and (2) by drying 

 in an air bath (ioo-uo). 



(e) Lipins (material extracted with ether) : The substance was weighed 

 in cone-shaped filter paper thimbles. The thimbles were suspended by 

 means of wires to reflux condensers. These were fitted with flasks con- 

 taining ether. After 24 hours of hot extraction the thimbles containing 

 the extracted bacteria were dried in vacuo to constant weight. The 

 difference in the weight of the thimble before and after extraction rep- 

 presents the weight of lipins. 



(f) Blanks: Blank determinations on the reagents used were made in 

 all cases. In the analyses for N the accuracy of the method for the small 

 quantities obtained was checked by estimating the N recovered from 

 known quantities of pure salt (ammonium sulphate) . 



2. RECORDS OF ANALYSIS: 



(a) Organism No. 688: 



Nitrogen: 0.0199 gram material gave 0.0019 gram N = 9.55 



per cent N; 0.0217 gram material gave 0.0022 gram N = 10.13 



per cent N. 

 Sulphur: 0.5336 gram material gave 0.0247 gram BaSO 4 = 0.63 



per cent S; 0.5769 gram material gave .0259 gram BaSO 4 = 0.61 



per cent S. 

 Phosphorus: 0.5336 gram material gave 0.0393 gram Mg 2 P 2 O7 = 



2.05 per cent P; 0.5769 gram material gave 0.0432 gram 



Mg 2 P 2 O 7 = 2.08 per cent P. 

 Ash: 0.5354 gram material gave 0.0526 gram Ash = 9.82 per 



cent Ash; 0.4694 gram material gave 0.0458 gram Ash = 9.75 



per cent Ash. 

 Moisture: A. Determined by drying the bacterial material in 



vacuo: 0.066 1 gram material gave 0.0485 gram H 2 O = 73.37 



per cent; 0.0866 gram material gave 0.0636 gram H 2 O = 73.44 



per cent. B. Determined by drying the bacterial material 



at 1 00 C: 0.0899 gram material gave 0.0671 gram H 2 O = 



74.63 per cent; 0.0769 gram material gave 0.0573 gram H 2 O 



= 74.52 per cent. 

 Lipins: 0.3073 gram bacterial material gave 0.0047 gram Lipins 



= 1.53 per cent; 0.3343 gram bacterial material gave 0.0055 



gram Lipins = 1.64 per cent. 



(b) Organism No. 720: 



Nitrogen: 0.0257 gram material gave 0.0026 gram N = io.ii per 

 cent N; 0.0243 gram material gave 0.0025 gram N = 10.28 

 per cent N. 



17 



