3. SUMMARY OF THE DATA FOR THE AVERAGE PERCENTAGE COMPOSI- 

 TION OF THE STREPTOCOCCI. 



4. CONCLUSIONS FROM THE ABOVE ANALYTICAL DATA: The above 

 summary indicates the average results of the various analyses. It also 

 brings into comparison the figures for the composition of the different 

 streptococci and points out these facts: 



(a) There are no marked graded differences in either the nitrogen or 

 the sulphur content. It becomes apparent, therefore, that the total 

 amounts of protein material in the different organisms varies but slightly, 

 if at all. 



(b) The phosphorus content is practically constant. This indicates no 

 striking variations in the quantity of nucleoprotein (the phosphorus due 

 to phosphatide being very small in amount) . 



(c) Regarding the results for the moisture determinations only slight 

 deviations are noticed. The percentages obtained by drying the sub- 

 stance at iooC. are higher than those obtained by drying the material 

 in vacuo. Such increases in the values were expected because of the 

 greater disintegration at the higher temperature. 



(d) Most interesting, however, are the figures obtained for the amounts 

 of Lipin material. These vary greatly for the different types of strep- 

 tococci. The percentage of lipins is lowest in the indifferent type and 

 highest in the green producing type, the hemolytic type taking a posi- 

 tion between these two. The characteristic relations described above 

 may be expressed by the ratio: 10 : 29 : 41 ; or briefly : 



Indifferent: 



Hemolytic: Green-producing 

 3:4 



These results separate the streptococci into three groups corre- 

 sponding to those obtained when the reaction on blood media is used as 

 the criterion. 



Because of the apparent significance of this relationship, we repeated 

 this part of the investigation. Fresh cultures were amassed and dried 

 as described above. A series of nine organisms were used, three from 

 each representative group, the strains 688, 720, 904, 1210, 863 and 1074 

 being included . The three new strains were chosen at random from our 

 stock cultures of indifferent, green-producing and hemolytic streptococci. 



These results were obtained: 



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