Designation of Organism 



Number Type Average Percentage Lipins 



688 Indifferent 1.46 



720 Indifferent 1 .54 



725 Indifferent 1.16 



904 Hemolytic 3.16 



1210 Hemolytic 3.73 



1012 Hemolytic 3-79 



863 Green-producing 5.31 



1074 Green-producing 5.34 



953 Green-producing 5.68 



A mere glance at the table brings to light the possible ratio: 



1:3:4 

 This similates the data gathered from our first group of determinations. 



V. ANALYSIS OF THE LIPIN MIXTURE 



1. OUTLINE FOR THE WORK: The very striking regularity in the 

 amounts of Lipin material and the fact that the groups of streptococci 

 parallel those obtained by the blood media reaction suggested the possible 

 relation between the nature of the Lipin mixture and the hemolyzing 

 capacity of the organisms. In an investigation of this character the chief 

 factors to be considered are (i) the phosphatide content, (2) the choles- 

 terol content and (3) the ratio between the phosphatide and cholesterol. 



A preliminary qualitative examination of the material showed that all 

 the extracts contained both cholesterol and phosphatide. These sub- 

 stances were then determined quantitatively by nephelometric methods 

 (65,66,67,68). 



2. RECORDS OF ANALYSIS OF THE LIPIN MIXTURE. 



(a) Organism No. 688: 



Phosphatide: 2.3560 gram bacterial material gave 0.0400 gram 

 Lipins which yielded 0.0000125 gram H 3 PO 4 = 0.25 per cent 

 Phosphatide; 2.4960 gram bacterial material gave 0.0380 gram 

 Lipins which yielded 0.0000125 gram H 3 PO4= 0.25 per cent 

 Phosphatide. 



Cholesterol: 2.5360 gram bacterial material gave 0.0400 gram 

 Lipins which yielded 0.00064 gram Cholesterol =1.6 percent 

 Cholesterol; 2. 4960 gram bacterial material gave 0.0380 gram 

 Lipins which yielded 0.00064 gram cholesterol = 1.7 per cent 

 cholesterol. 



(b) Organism No. 720: 



Phosphatide: 2.4411 gram bacterial material gave 0.0290 gram 

 Lipins which yielded 0.0000087 gram H 3 PO 4 = 0.24 per cent 

 Phosphatide; 2.4301 gram bacterial material gave 0.0280 

 gram Lipins which yielded 0.0000087 gram H 3 PO 4 = 0.24 

 per cent Phosphatide. 



Cholesterol: 2.4411 gram bacterial material gave 0.0290 gram 

 Lipins which yielded 0.00046 gram Cholesterol = 1.5 per cent 

 2.4302 gram bacterial material gave 0.0280 gram Lipins which 

 yielded 0.00046 gram Cholesterol =1.5 per cent. 



(c) Organism No. 904: 



Phosphatide: 1.7090 gram bacterial material gave 0.0710 gram 

 Lipins which yielded 0.000006 gram H 3 PO 4 = 0.064 per cent 

 Phosphatide; i .6980 gram bacterial material gave 0.0640 gram 



