The results of this experiment indicate that in the absence of choles- 

 terol 0.08 c.c. of a 0.2% saponin solution is the smallest amount that 

 produces hemolysis with i.o c.c. cell suspension in 8 minutes. This is, 

 therefore, the test dose and was used in determining the effect of the 

 ether extract on the action of saponin. 



The lipins extracted from the bacteria were dissolved in definite volumes 

 of ether: three-fifths of this solution was evaporated to a volume of 3cc. 

 and then emulsified with 27 cc. of 0.9% NaCl solution. Graded amounts 

 of this emulsion were added to the various tubes. 



Experiment (c): To determine the effect of the extract from organism 

 no. 688 on the action of saponin. 



Incubation at 25 for 15 Minutes 



Experiments (d), (e), (f), (g), (h) were performed respectively with 

 extracts from organisms 720, 904, 1210, 863, 1074. The results parallelled 

 exactly those of experiment (c). 



As checks on the validity of the above experiments controls were run 

 on the various ingredients: 



No. 



i 



2 



3 

 4 

 5 

 6 



7 

 8 



9 

 10 



Combination 



Salt solution and cell suspension 

 Lecithin and cell suspension 

 Cholesterol and cell suspension 

 Saponin and cell suspension 

 Extract 688 and cell suspension 

 Extract 720 and cell suspension 

 Extract 904 and cell suspension 

 Extract 1210 and cell suspension 

 Extract 863 and cell suspension 

 Extract 1074 and cell suspension 



Hemolysis 

 o 

 o 

 o 



4-4-4- + 

 o 

 o 

 o 

 o 

 o 

 o 



2. CONCLUSIONS FROM DATA ON HEMOLYSIS. 



(a) The lipin mixtures from the various organisms show the same 

 effect on the action of saponin they inhibit hemolysis. 



(b) The differences, mentioned above, in the ratios of cholesterol to 

 phosphatide cannot be correlated with differences in the action of the 

 ether extracts on the hemolytic power of saponin. 



