VII. SUMMARY OF CONCLUSIONS. 



1 . There are no marked graded differences in either the N or S content. 

 It becomes apparent, therefore, that the total amounts of protein material 

 in the different organism varies but slightly. 



2. The P content is practically constant. This indicates no striking 

 variations in the quantity of nucleoprotein (the P from the phosphatide 

 being very small in amount). 



3. Regarding the results for the moisture determinations only slight 

 deviations are noticed. The percentages obtained by drying the material 

 at 100 C. are higher than those obtained by drying the substances in 

 vacuo. Such increases in the values were expected because of the greater 

 disintegration at the higher temperature. 



4. Most interesting are the figures obtained for the amount of lipin 

 material. These vary greatly for the different types of streptococci. 

 The percentage of lipins is lowest in the indifferent type and highest in 

 the green-producing type, the hemolytic type taking a position between 

 these two. The characteristic relations may be expressed by the ratio: 

 Indifferent: Hemolytic: Green Producing:: 1:3:4. These results seem 

 to seperate the streptococci into three groups corresponding to those 

 obtained when the reaction on blood is used as the criterion. 



5. The content of phosphatide in the lipin mixture decreases for the 

 different types in the order named: indifferent, hemolytic, green-pro- 

 ducing. 



6. The same relation is given by the amounts of cholesterol contained 

 in the different organisms. 



7. The ratio C:P increases when the types are listed as above. 



8 . The lipin mixtures from the various organisms show the same effect 

 on the action of saponin: they inhibit hemolysis. 



9. The above mentioned differences in the ratios of cholesterol to 

 phosphatide cannot be correlated with differences in the action of the 

 ether extracts on the hemolytic power of saponin. 



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