These figures were chosen as examples because the test materials 

 matched these most often in the colorimetric determinations. 



(b) Indicators used: The indicators used were those recommended by 

 Clark and Lubs and manufactured by Hynson, Westcott and Dunning, 

 Baltimore. The following table shows the character of the indicator 

 solutions and notes the amounts of the several varieties used: 



4. ISOLATION OF THE STRAINS: The fresh material taken from the root 

 canal was planted on hemolyzed sheep blood-glucose-veal infusion agar 

 slants (0.2 c.c. sterile hemolyzed defibrinated blood plus 0.5 c.c. 12% 

 sterile glucose solution for each 6 c.c. medium) to which o.i c.c. sterile 

 i% glucose-beef-infusion broth had been added after solidification. 

 After 1 8 to 20 hours incubation at 37 C., a loopful was streaked on several 

 whole sheep-blood glucose infusion agar plates. Single colonies fished 

 from these plates were inoculated into glucose infusion broth. If micro- 

 scopic examination proved this a pure streptococcus culture, transplants 

 were made to whole sheep blood infusion agar (0.5 c.c. blood plus 9.5 c.c. 

 agar) and ox bile. The reactions obtained for 70 cultures used as stock 

 strains are given in the following table: 



Strain 

 852 



853 

 863 

 874 



877 

 882 

 894 

 904 

 909 

 911 

 922 

 948 

 953 

 956 

 961 



Reaction to 



Whole Blood 

 I 

 I 



G 

 I 

 I 

 I 

 I 



H 

 I 

 I 

 I 

 I 



G 

 I 

 I 



Reaction to 

 Whole Blood 

 I 



I 

 I 

 G 



I 



I 



I . 



G 



G 



I 



I 



I 



G 



G 



I 



Bile 

 O 



