2. Cultures 



(a) Preparation of the cultures for mass growing 



(b) Planting of cultures 



(c) Removal of growth from agar 



(1) Washing method 



(2) Spatula method 



(d) Drying of growth 



IV. Analysis of the Bacterial Material 



1 . Description of methods employed 



(a) Nitrogen 



(b) Total sulphur and phosphorous 



(c) Ash 



(d) Moisture 



(e) Lipins 



(f) Blanks 



2. Records of analysis 



3. Summary of the data for the average percentage composition 

 of the streptococci 



4. Conclusions from the analytical data 



V. Analysis of the Lipin Mixture 



1 . Outline of the procedure 



2. Records of analysis 



3. Summary of the data for the average percentages of phosphatide and 

 cholesterol. 



4. Conclusions from the data on the cholesterol and phosphatide content 



VI. Effect of the Lipin Material on Hemolysis 



1 . Record of experiments 



2. Conclusions from the data on hemolysis 



VII. Summary of Conclusions 

 VIII. Bibliography. 



