CONTENTS 



I. Introduction 



1 . General Statement 



2. Historical 



3. Object of Research 



II. Character of Organisms 



1 . Source 



2. Preparation of media for use in the determination of 

 the biochemical reactions. 



(a) Sugar-free broth 



(b) Carbohydrate broth 



(c) Meat infusion agar 



(d) Meat infusion gelatin 



(e) Milk 



(f) Inulin serum water 



(g) Bile 



3. Preparation of the reagents for the determination of 

 the hydrogen-ion concentration. 



(a) Standard solutions 



(b) Indicators 



4. Isolation 



5. Stock Cultures 



6. Seed Cultures 



7. Biology and Biochemistry of the organisms 



(a) Blood reactions 



(b) Immunity reactions 



(c) Other biochemical reactions 



8. Description of the test organisms 



III. Preparation of the Mass Cultures 

 I . Medium 



(a) Choice of the Infusion basis 



(1) Hemolyzed blood- veal-infusion agar 



(2) Huntoon agar 



(3) Hasting gelatin-agar 



(4) Beef liver agar 



(5) Beef brain agar 



(b) Concentration of agar agar 



(c) Constancy in composition 



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