BLOOD. 



IS 



y-band is much more diffuse than the oxyhemoglobin bands, and its limits 

 therefore, especially in weak solutions, are not well defined; in solutions 

 of blood diluted 100 times with water, which would give a haemoglobin 

 solution of about 0.14 per cent., the absorption band lies in the part of the 

 spectrum included between the wave-lengths X 572 and X 542. The width 



70 65 



B C 



E b 



Fig. 5.— Diagrammatic representation of the absorption spectrum of haemoglobin (reduced haemoglo- 

 bin) (after Rollett). The numerals give the wave-lengths in hundred-thousandths of a millimeter ; the 

 letters show the positions of the more prominent Fraunhofer lines of the solar spectrum. The red end 

 of the spectrum is to the left. The single diffuse absorption band lies between d and e. 



and distinctness of this band vary also with the concentration of the 

 solution. This variation is sufficiently well shown in the accompanying 

 illustration (Fig. 6), which is a companion figure to the one just given 

 for oxyhemoglobin (Fig. 4). It will be noticed that the last light to 

 be absorbed in this case is partly in the red end and partly in the blue, 

 thus explaining the purplish color 

 of hemoglobin solutions and of 

 venous blood. Oxyhemoglobin so- 

 lutions can be converted to hemo- 

 globin solutions, with a correspond- 

 ing change in the spectrum bands, 

 by placing the former in a vacuum 

 or, more conveniently, by adding 

 reducing solutions. The solutions 

 most commonly used for this pur- 

 pose are ammonium sulphide and 

 Stokes's reagent. 1 If a solution of 

 reduced hemoglobin is shaken with 

 air, it quickly changes to oxyhemo- 

 globin and gives two bands instead 

 of one when examined through the 

 spectroscope. Any given solution 

 may be changed in this way from 

 oxyhemoglobin to hemoglobin, 

 and the reverse, a great number 

 of times, thus demonstrating the 

 facility with which haemoglobin 

 takes up and surrenders oxygen. 



1 Stokes's reagent is an ammoniacal solution of a ferrous salt. It is made by dissolving 2 

 parts i by weighl ) of ferrous sulphate, adding ."> parts of tartaric acid, and then ammonia to dis- 

 tinct alkaline reaction. A permanent precipitate should not be obtained. 



Fig. (i.— Diagram to show the variations In the ab- 

 sorption spectrum of reduced haemoglobin with vary- 

 ing concentrations of the solution (after Rollett). The 

 numbers to the right give the strength <>t' the haemo- 

 globin solution in percentages ; the Letters give the posi- 

 tions of the Fraunhofer Lines. For further directions 

 as in the use of the diagram, see the description of 

 Figure 1. 



