266 



BOTANICAL GAZETTE 



[OCTOBER 



wheat and other small seeds during the first stages of germination; 

 but if the experiments are to be carried beyond this stage, the seed- 

 lings must be supported so that the leaves do not come into contact 



with the solution. After 

 some trials the following 

 device was hit upon which 

 answers the purpose 

 admirably. A strip of 

 filter paper is folded 

 lengthwise and one of 

 the folds turned back as 

 shown in fig. 5. The 

 seeds are placed in the 

 trough thus formed and 

 the whole strip is then 

 bent into a circle and 

 placed in a tumbler previ- 

 ously filled with solution. 

 The strip should be of 

 such length that when 

 placed in the top of the 

 tumbler the ends just 

 meet and so form a stiff 

 collar which just fits 

 inside the top of the 

 tumblers and which will 



FIG. 4. Development of spores 

 of Equisetum in various 3^/160 

 solutions during 50 days. Quanti- 

 ties are stated in cubic centi- 

 meters; the gain in length of 

 thallus exclusive of rhizoids is 

 stated in per cent. I, distilled 



water, gain 1760. 2, dilute arti- 

 ficial sea water, NaCl = ap. 

 3w/i6o, gain 1500. 3, NaCl 

 1000 + KC1 22 -f Cada 10, gain 

 1500. 4, NaCl iooo+CaCl 2 10, 

 gain 980. 5, CaCl 2 , gain 700. 

 6, NaCl, gain o. 



not slip down. A large 

 number of these collars 

 may be prepared, filled 

 with seeds, bent into 

 circles, and secured by 

 ordinary paper-clips 



placed on the overlapping ends. They may be piled in trays until 



wanted. It is then only necessary to remove the clips and set the 



collars in glasses previously filled with solutions. 



In some cases, especially where larger glasses are employed, a 



strip of paper of double thickness may be used ; this makes a stiff er 



