1907] 



OSTERHOUT BALANCED SOLUTIONS 



263 



The gemmae were removed from the cups with the point of a 

 knife and sprinkled on the surface of the solution, care being taken 

 to exclude particles of dust and dirt. Each tumbler contained 2oo cc 

 of solution and was covered with a glass plate. The gemmae may 

 be easily removed from the surface of the solution for purposes of 

 observation by dipping into it a clean slide and slowly raising it at an 

 angle so as to take up the material upon one side only. The material 

 should not be replaced in the solution unless extreme care be taken 

 to prevent contamination. 



The average results of six series of experiments are given in Tables 

 IV and V, and illustrated in figs. 2 and j. 



TABLE IV, LUNULARIA 



A plus sign indicates that the plants were alive when the experiment was stopped. 

 All quantities given are cubic centimeters of 3^/32 solutions. 



CULTURE SOLUTION 



Dilute sea water; total salts 



0.6 per cent 



Dilute artificial sea water: 

 1000 NaCl 

 78 MgCl 2 

 38 MgS0 4 

 22 KC1 

 10 CaCl 2 



Distilled water. . . . 

 NaCl. . . 

 1000 NaCl 

 10 CaCl 2 



1000 NaCl 

 22 KC1 

 10 CaCl 2 



1000 NaCl 

 78 MgCl 2 

 10 CaCl 2 



1000 NaCl 

 78 MgCl 2 

 22 KC1 



1000 NaCl 

 22 KC1 



1000 NaCl 

 78 MgCl 2 

 MgCl 2 . 

 KC1 .. 

 CaCl 2 .. 



DURATION OF LIFE IN DAYS 



200 + 



200 + 



2OO + 



4 



200 + 



2OO + 



2 



12 

 100 



