194 MR. NEWPORT ON THE IMPREGNATION OF 



made, and to detail them when considering the subject to which they more especially 

 refer. 



No. 1 . P.M. 2 U 52 m . Eighty-three ova were passed into water, and the impregnating 

 fluid added to them at the expiration of one minute. This was forty-three minutes 

 after the fluid had been obtained and mixed with water; but on examination with 

 the microscope at this period, the spermatozoa contained in it were still very active. 

 This long period was determined on with a view to the more effectually testing the 

 susceptibility of the ovum, as it will be shown that the spermatozoa are less and less 

 efficient in proportion to the length of time they have been mixed with water. Seg- 

 mentation commenced in a very large proportion of the ova at the end of three hours 

 andjifty-jive minutes. On the 14th of April, the eighth day, thirty-two embryos had 

 been formed. 



No. 2. P.M. 2 1 ' 51 m . Ninety-two ova were passed into water, and at the expiration 

 of two minutes impregnating fluid was added to the same, forty-three minutes after it 

 had been obtained. Segmentation commenced in these also at three hours and Jifty -five 

 minutes, and took place in almost every ovum. On the eighth day there were forty- 

 five embryos. 



No. 3. P.M. 2 h 24 m . One hundred and twenty-seven ova were immersed in water 

 for three minutes, and then exposed and bathed with impregnating fluid during 

 twenty seconds, water being immediately afterwards added to them. No segmentation 

 had taken place at the end of four hours and three minutes, but it took place in manv 

 of the ova at a later period, the exact time having escaped my notice. The fluid 

 employed had been mixed with water only seventeen minutes. On the eighth day 

 there were thirty-three embryos. 



No. 4. P.M. 2 h 15 m . Eighty-one ova were exposed to the air on a dry surface for 

 three minutes without having been in contact with water, and were then bathed with 

 impregnating fluid during Jive seconds and water immediately afterwards added to 

 them. The fluid in this experiment had been obtained only eight minutes. Segmenta- 

 tion commenced in several ova at four hours and Jive minutes, and on the eighth day 

 there werefifty-three embryos. 



No. 5. P.M. 2 h 25 m . One hundred and thirty-six ova were passed into water for Jive 

 minutes, and were then exposed and bathed with impregnating fluid for several seconds, 

 and water immediately afterwards added to them. The fluid had been obtained 

 twenty minutes. Segmentation occurred in one ovum at four hours and eight minutes, 

 and in others quickly after. On the eighth day only ten embryos had been formed. 



No. 6. P.M. 2 h 17 m . One hundred and thirty-nine ova were exposed to the air, on 

 a dry surface, for Jive minutes, and were then touched freely with fluid during Jive 

 seconds, applied with a hair-pencil, and water was then quickly added to them. The 

 fluid employed had been obtained twelve minutes. Segmentation took place in four 

 hours and eleven minutes. On the eighth day there were thirty-seven embryos. 



No. 7. P.M. 2 h 21 m . Two hundred and Jive ova were retained in water for fifteen 



