

CRYSTALLOID IN LIVING CELLS 323 



undergone a further increase above that which they possessed 

 when in union with the haemoglobin, and now lies at about one- 

 half the value in the serum. Even dialysis, however, is unable 

 to detach the phosphates from the haemoglobin, and the above 

 conductivity of one-half is chiefly due to chlorides detached in 

 the process of dialysis. It is only after incineration and making 

 up to original volume that the conductivities of corpuscles and 

 serum become practically equal. 



The following table illustrates these interesting changes in 

 conductivity accompanying detachment of colloid and crystalloid 

 in two experiments on separated blood-corpuscles and serum. 

 The figures give specific conductivity multiplied by 10 5 to save 

 decimals. 



SAMPLE I. SAMPLE II. 



Treatment to which subjected. Serum. Corpuscles. Serum. Corpuscles. 



1. Fresh 1705 95 1519 109 



2. Frozen solid and thawed\ , Ang o in use oq^ 



(corpuscles laked) ../ 



3. Dialysed and volume re-j Ig43 g91 1623 m 



duced to original . . J 



4. Incinerated and ash made\ 160g 16?7 169? 1655 



up to original volume . . J 



That the phosphates are more firmly held than the chlorides, 

 so that the union persists even in presence of a very low concen- 

 tration of phosphatic ions in the fluid, is shown by the following 

 analysis for chlorides and phosphates in the dialysates of corpuscles 

 and serum respectively, and in the two cases after incineration. 

 The figures also illustrate the very different distribution of chlorides 

 and phosphates in corpuscles and serum respectively. 



Serum Percentages. Corpuscle Percentages. 



Cl. P 2 5 . Cl. P 2 5 . 



Dialysis .. 0-3657 0-0197 0-1331 0-0329 



Incineration .. 0-3373 0-0219 0-1704 0-1708 



Even the chlorides are more strongly held in corpuscles than in 

 serum, the figure on incineration being considerably higher than 

 after dialysis, 1704 as against 0-1331, instead of being slightly 

 lower, due to volatilisation with organic matter, as in the case of 

 the serum, 0-3373 as against 0-3657. In the serum the phosphate 

 figures are almost equal by the two methods, but in the corpuscles 

 the evidence of union is clear, only 0-0329 per cent, dialyses out of 



