vru 



little bit thus prepared in a hole scooped out in solid paraffin. Melt a little paraffin in a small spoon and pour 

 around the specimen. 



Section-cutting. Wet razor with alcohol by means of a brush. Cut a number of slices and transfer 

 them with the brush to a watch-glass. Add water, the gum dissolves, and the sections become detached from 

 the paraffin. 



PERN. 



Sections. The rhizome cuts better after steeping for some time. 



Staining. A transverse section of fibre-vascular bundle, for instance, consisting of hard and close-set 

 cells, may be stained by soaking with magenta for some time in a watch-glass, then washing with water from a 

 pipette before mounting. 



FLOWERING PLANT. 



Leaf. Cut a small piece ont of blade of leaf. Dip it into gum and then into alcohol. Imbed in paraffin 

 and cut transverse sections. 



Peel off a small bit of epidermis from the lower surface, say, of a geranium leaf, and examine in water. 



Anther. Take anther from a flower before it opens. Dip in gum and alcohol before imbedding and 

 cutting sections. 



Ovule. One of the Speedwells may be used, e.g. Buxbaum's Speedwell. Dissect out ovules from ovary of 

 flower from which corolla has just fallen, then tease them asunder under dissecting microscope in a drop of 

 potash solution and add a little glycerine when mounting. 



Embryo. The common Shepherd's Purse may be used. Dissect out the mature ovules or seeds from 

 fruit. Commence with youngest fruit nearest the top, afterwards going to older, to get different stages. Mount 

 in potash solution, press gently with cover-glass, and the embryo will be forced out through the micropyle. 



Seeds. Beans or Peas are readily examined after steeping for a night in water. 



Germination. Wheat and Indian Corn can be readily germinated by placing in moist tow, surrounded by 

 flannel, and kept in a warm place. The root hairs and root cap can be readily seen on these young roots. 



AMCEBA. 



Examination. The readiest place to find some form or other of Amoeba is in the slimy matter taken from 

 decaying leaves in stagnant water. Mount in a drop of water and examine like Yeast. 



COLOURLESS BLOOD-CORPUSCLES. 



Examination. Lay a drop of your own blood on a slide, then cover quickly with cover-glass. By means 

 of a camel-hair brush surround with oil to prevent evaporation. 



VORTICELLA. 



Examination. On minute Water-plants, such as Duck-weed, groups are usually found. The operation of 

 feeding may be observed by introducing particles of indigo or carmine into the water. 



HYDRA. 



Examination. Found in stagnant pools and ditches. To examine alive, mount in plenty of water under 

 a large cover-glass. 



Sections. Kill by adding boiling water. Harden, prepare, and imbed as in Chara. 

 Sea-anemones treated in the same way might be used instead. 



EARTH-WORM. 



Dissection. Kill with vapour of chloroform by placing in a stoppered bottle, with a few drops of chloro 

 form. It then becomes stretched, and may be dissected under spirit. Fasten out in a dissecting dish, and 

 with a fine pair of scissors slit it up longitudinally a little to one side of the middle line and pin out the two 

 flaps. Needles with borax leads for heads are best for pinning out. 



