Upon the Pseudo- Diphtheria, etc. 37 



subsided, and no further attention was paid to the matter. 

 During May, 1897, there was a return of the symptoms, 

 but in milder form. On examination the same organism 

 was again found in the urine. It was found repeatedly for 

 over a month. Whether the organism really came from 

 the bladder or not it is impossible to state. It was found 

 in cultures made from the meatus, but, judging from the 

 symptoms, I am inclined to believe it was present in the 

 bladder itself. Careful study of the organisms isolated 

 from my urine in May revealed no biological or morpho- 

 logical differences from the organisms isolated in January. 

 Growth always took place most readily at the body tem- 

 perature ; though after growth had once become established 

 on ordinary media it was found possible to grow the organ- 

 ism at the room temperature. Morphologically, when 

 grown on agar-agar or blood serum the organism appeared 

 as a short, slender rod, sometimes showing a tendency to 

 clubbing at the ends, which stained more deeply at the 

 ends than elsewhere ; usually, when stained with dilute 

 methylene blue, the bacilli were marked with alternate 

 bands of deeply-stained and unstained areas. Usually, the 

 longer and more club-shaped organisms showed the striae 

 best. It was never seen to grow visibly on potato. In 

 litmus-milk there was sometimes a change to a deeper blue 

 color. There was not not always a growth on gelatin. 

 The color of the rosolic acid solution always remained un- 

 changed. In bouillon there was at times a very slight 

 flocculent sediment at the bottom of the tubes. On blood 

 serum no coalescence of the small round colonies was ob- 

 served at any time. The bacilli appeared to stain best 

 with aqueous methylene blue, but also stained well with 

 Loffler's alkaline methylene blue. They stained moder- 

 ately well with aqueous fuchsin ; and more deeply with 

 carbol-fuchsin. They stained deeply by Gram's method. 

 By staining lightly with aqueous methylene blue and 

 counterstaining with Bismarck brown, small bluish gran- 



