Upon the Pseudo- Diphtheria, etc. 41 



along the sides and at the bottom of the tube, but no 

 mycoderm. 



When grown in litmus-milk considerable variation was 

 noticed with regard to the reaction of the milk after grow- 

 ing at 34 C. for ten days. Under the same conditions 

 cultures 13, 14, 19, 23, 25, 26, 27 and 29 produced an 

 alkaline reaction which was noticeable in most instances 

 after twenty-four hours, while cultures 5, 15, 16, 18, 20, 21 

 and 24 produced an acid reaction which was noticeable 

 only after growing for four days. 



When grown in neutral litmus glucose bouillon more 

 satisfactory results were obtained with regard to the pro- 

 duction of acids, by the different cultures, than with the 

 use of litmus milk. With the exception of cultures 23, 

 26, 27 and 29, all produced an acid reaction in this medium, 

 after growing in the incubator for ninety-six hours. Some 

 of the cultures showed a slight acid reaction after seventy- 

 two hours, while culture 20 showed a marked acid reaction 

 after twenty-four hours' growth. The extent of acid pro- 

 duction by this culture was apparently equal to that of 

 two cultures of the I/5ffler bacillus grown under the same 

 conditions. Cultures 21 and 31 produced almost the same 

 degree of acidity as the cultures of the Loffler bacillus, 

 though not as rapidly as in the case of culture 20. Cul- 

 tures 23 and 29 failed to change the reaction of the medium. 

 Cultures 26 and 27 showed a distinct alkaline reaction 

 after growing ninety-six hours. These were the only cul- 

 tures which produced an alkaline reaction in this medium. 



When grown on potato, cultures 5, 18, 20, 21, 23, 24, 26 

 and 29 showed a slight growth after ten days at 34 C. A 

 similar growth was noticed in cultures of the Loffler 

 bacillus when grown under the same conditions. With 

 the exception of cultures 20 and 21 the growth was barely 

 perceptible. It is probable that the potato was somewhat 

 alkaline, as the Loffler bacillus does not grow on acid 

 potato. 



